Antimicrobial activities and phytochemical analysis of the. The basil essential oil presented antibacterial activity in all tested bacteria, except for pseudomonas aeruginosa. The ocimum basilicum oil recorded the highest aeac, which surpassed vitamin es aeac under the same experimental conditions. Ethanol, methanol, and hexane extracts from ocimum basilicum labiatae sweet basil were investigated for their invitro antimicrobial properties. The plant grows in several regions around the world. Results obtained from discdiffusion method, followed by measurements of mic values, indicated that the eo of l. Shaukat1 1department of chemistry, government postgraduate college for women gulberg, affiliated with lahore college. Preparation of beef burger five kg beef burger was taken from a batch production of a meat products factory. Study of pharmacological effect of ocimum basilicum.
Antioxidant and antibacterial activity of basil ocimum. International science congress association 291 antibacterial activity of leaf extracts of ocimum sanctum l. The results showed that one rosmarinic acid can capture 1. Antibacterial activity of basil essential oil ocimum. Larvicidal and repellent activity of vetiveri azizanioides l, ocimum basilicum l and the microbial pesticide spinosad against malarial vector, anopheles 76 stephensi liston insecta. Antimicrobial properties of basil and its possible. Besides their antioxidant activity, the antimicrobial effect of these extracts confirms the biological activities of these herbal medicinal products. Basil is the common name for the culinary herb ocimum basilicum of the family lamiaceae labiatae. Phytochemical constituents and antimicrobial and grain protectant activities of clove basil ocimum gratissimum l. Antibacterial and antioxidant study of ocimum basilicum. Essential oil isolated from five plant varieties growing in. Both the hexane and ethanol extracts, but not the ethanol extracts, inhibited three isolates out studied. Pdf on sep 2, 2016, malgorzata nabrdalik and others published antibacterial activity of ocimum basilicum l.
Q s1, pessoa h l f2, freitas a f r2, alves l b n3, lima e o4 1graduate program in synthetic and natural bioactive products, paraiba federal university, 58051900, joao pessoa, paraiba, brazil. The experiment was run in five replicates with the following concentrations of the essential oil from o. Characterization of the volatile composition of essential. Antibacterial activity of ocimum basilicum essential. Antibacterial activity of leaf extracts of ocimum sanctum. Antimicrobial activity of essential oil extract of ocimum.
In which 15ml of sterile muller hinton agar was poured into various petri dishes, they were allowed to solidify. Studies of antimicrobial properties of different leaf. Jun 21, 2019 the ocimum basilicum oil recorded the highest aeac, which surpassed vitamin es aeac under the same experimental conditions. In this study the glandular hair morphology, chemical composition and antimicrobial activity of the essential oils from three ocimum species have been investigated ocimum basilicum l. Antimicrobial activities of ocimum basilicum extract this test was carried out according to the method of 9. The antioxidant activity was evaluated as a free radical scavenging capacity rsc, together with effects on lipid peroxidation lp. For centuries, plants have been used for a wide variety of purposes, from treating infectious diseases to food preservation and perfume production. The screening of antimicrobial activity of the tested extracts samples was evaluat.
Antimicrobial and free radical scavenging activities of basil ocimum basilicum. The leaves are ovate, lanceolate, cucuminate, toothed or entire, glabrous on both surfaces and glandular. To assess the antimicrobial activity of ocimum basilicum rehan leaf extract against human pathogenic bacterial pathogens. Antimicrobial activity of various extracts of ocimum. Ocimum basilicum at the concentration of 500 gml showed 75. Forty two volatile compounds were identified by using gas chromatographymass spectroscopy gcms. The antimicrobial activity from mikania triangularis, known as thin leaf guaco, was tested against five genera of bacteria and three genera of. Biosynthesis of zno nanoparticles using ocimum basilicum and determination of its antimicrobial activity s. Grown in nigeria abdullahi mann department of chemistry, federal university technology, minna, p. Antimicrobial activity for antimicrobial testing, methanol, dichlormethane and cyclohexane extracts were used. Basil is the common name for the culinary herb ocimum basilicum l. The antimicrobial activity of plant extracts and phytochemicals was evaluated with antibiotic susceptible and.
Evaluation of antibacterial activity of ocimum basilicum on. Yamani ha, pang ec, mantri n and deighton ma 2016 antimicrobial activity of tulsi ocimum tenuiflorum essential oil and their major constituents against three species of bacteria. Antimicrobial activity of basil ocimum basilicum oil against salmonella enteritidis in vitro and in food pongsak rattanachaikunsopony and parichat phumkhachorn department of biological science, ubon ratchathani university, warin chamrap, ubon ratchathani 34190, thailand. Chemical composition, antioxidant and antimicrobial activities of the essential oils from aerial parts of basil ocimum basilicum l. The composition and antibacterial activity of essential.
The indumentum shows little variation among the investigated species with both glandular and nonglandular. The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of ocimum basilicum l. Phytochemical constituents and antimicrobial and grain. This is justified by the relevance of obtaining safe products, with emphasis on the use of natural additives. Basil essential oils and their principal constituents were found to exhibit antimicrobial activity against a wide range of gramnegative and grampositive bacteria, yeast, and mold. Introduction medicinal plants essentially contain active constituents that are used in the treatment of many human diseases. Antimicrobial activity of basil, oregano, and thyme essential oils. Isolation of potential antibacterial and antioxidant. Studies of antimicrobial properties of different leaf extract. The antimicrobial activity of ocimum basilicum was carried out according to the method described by joshi et al. Antibacterial activity of ocimum basilicum essential oil and linalool on bacterial isolates of clinical importance.
Antimicrobial activity, disc diffusion method, ocimum basilicum, plant extract. Pdf antimicrobial activity of essential oil extract of ocimum. Ethanol, methanol, and hexane extracts from ocimum basilicum labiatae sweet basil were investigated for their in vitro antimicrobial properties. Based on the size of the inhibition zone and the minimal inhibitory concentration, basil oil had the strongest antimicrobial activity against all the tested bacteria, and s. Chemical composition and antimicrobial activities of leaves of sweet.
Aromatic profiles and antimicrobial activities of two. Ocimum basilicum plant classification of ocimum basilicum basil ocimum basilicum of the family lamiaceae the plant tastes somewhat like anise, with a strong, pungent, sweet smell. Evaluation of antioxidant and antimicrobial activities and. This result may be attributed to the chemical composition of the o. Antibacterial activity of plant extracts 247 brazilian journal of microbiology 2000 31. Antimicrobial and free radical scavenging activities of basil. Figures uploaded by hercules sakkas author content. Antibacterial activity of ocimum basilicum semantic scholar. Tulsi ocimum tenuiflorum, essential oil, antimicrobial activity, headspacesolid phase microextraction, gas chromatographymass spectrometry. Apr 18, 2015 this study aimed to evaluate the antibacterial activity of basil essential oil in vitro and in italiantype sausage. The results indicated that the methanol extracts of o. Presently, the increasing resistance of microorganisms to currently used antimicrobials in combination with the appearance of emerging diseases requires the urgent development of new, more effective drugs.
The composition and antibacterial activity of essential oils. Antibacterial activity of ocimum basilicum essential oil and. Antibacterial activity antimicrobial activity of the essential oil from o. Antimicrobial activity of essential oil extract of ocimum basilicum l. The toxicity of extracts from arthemus sativa, which is known to have antimicrobial activity, was also studied 10. Antimicrobial activity of basil ocimum basilicum oil against salmonella enteritidis in vitro and in food pongsak rattanachaikunsopony and parichat phumkhachorn department of biological science, ubon ratchathani university, warin chamrap, ubon. George, in handbook of herbs and spices second edition, volume 1, 2012. Chemical composition and antibacterial properties of. Antimicrobial activities, essential oil and disc diffusion method, ocimum basilicum.
It has been observed that the extract exhibitted strong activity with the increase in polar solvent. Bioscience, biotechnology, and biochemistry 2010, 74 6, 12001204. Singh et al 20 has evaluated the qualitative estimation of phytochemicals and antimicrobial activity of aqueous and methanol extracts of root and leaves of ocimum sanctum against pathogenic bacteria e. Antibacterial activity of ocimum basilicum essential oil and linalool on bacterial isolates of clinical importance silva v a1, sousa, j p1, guerra f. The genus ocimum has a number of species that are used to treat different types of disorders and diseases from ancient times. Essential oils are rich sources of biologically active compounds and have been assessed to possess antimicrobial properties in vitro and in vivo 46. In contrast, less antimicrobial activity was obtained by 40 who tested the activity of the ethanol extracts of ten plants, including o.
Chemical composition and bioactivity of essential oils and. This study aimed to determine the chemical composition and antimicrobial activity of the volatile oil of egyptian ocimum basilicum l. Antimicrobial activity of basil ocimum basilicum oil against salmonella enteritidis in vitro and in food. Antimicrobial activity of basil, oregano, and thyme. May 17, 2016 in summary, the essential oil extracted from ocimum tenuiflorum showed antimicrobial activity against s. Pdf antimicrobial activity of basil, oregano, and thyme. Many of these plants which show antimicrobial activity are used in.
Antimicrobial activity of oregano origanum vulgare. Chemical composition, antioxidant and antimicrobial. Nine essential oils were examined for antimicrobial activity against reference and clinical strains of salmonella enteritidis. Determination of antimicrobial activity the antimicrobial activity of ocimum basilicum essential oil was assayed using microdilution method sharafatichaleshtori et al. The essential oils and extracts of plants have been reported to have an antimicrobial activity 3, 7. It was observed that the treated cells were damaged. Antibacterial activity of ocimum basilicum essential oil. Evaluation of antibacterial activity of ocimum basilicum. Antimicrobial activity of various extracts of ocimum basilicum l. This study aimed to determine the antibacterial activity of the major chemical components in the crude extract against methicillin resistant staphylococcus aureus mrsa isolated from the clinical specimens. The antioxidant activity of a methanolic extract of ocimum basilicum l. Extracts stanislava brdanin1, nemanja bogdanovic 1, marina kolundzic, marina milenkovic2, natasa golic3, milan kojic3, tatjana kundakovic1 1 department of pharmacognosy, faculty of pharmacy, university of belgrade. The antimicrobial activities of crude, ethanolic and methanolic extracts of ocimum basilicum ob basil plant against vibrio cholerae, a bacterial pathogen responsible for cholera were examined. Pdf antimicrobial activity of essential oil extract of.
The antimicrobial activities of crude, ethanolic and methanolic extracts of ocimum basilicum ob basil plant against vibrio cholerae, a bacterial pathogen. Secondary metabolites from ocimum species possess exceptional biological activity and have antioxidant 68 and antimicrobial 912, bactericidal 14, repellent 1516, anticonvulsant 17. The in vitro hypoglycemic activity of basil ocimum basilicum aqueous extract was investigated. Ocimum basilicum is an erect, almost glabrous herb, which grows to between 30 and 90 cm high. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. Antimicrobial activity of basil, oregano, and thyme essential. Aromatic profiles and antimicrobial activities of two ocimum. Enteritidis se3 was the most sensitive strain to all the tested oils. The hydrodistilled essential oils content ranged from 0. Antimicrobial activity of basil ocimum basilicum oil. Antimicrobial activity of oregano origanum vulgare l. Ocimum sanctum extract was found to be more effective on bacterial species. Mar 16, 20 in this study the glandular hair morphology, chemical composition and antimicrobial activity of the essential oils from three ocimum species have been investigated ocimum basilicum l. Composition and antimicrobial activities of lippia.
This study aimed to evaluate the antibacterial activity of basil essential oil in vitro and in italiantype sausage. It is universally cultivated as herbaceous, perennial plant but originated from asian continent. The indumentum shows little variation among the investigated species with both glandular and nonglandular hairs presents. The cells of microorganisms, which were treated and untreated with plant. Patcharee pripdeevech, watcharapong chumpolsri, panawan suttiarporn, sugunya wongpornchai. Antimicrobial activity of tulsi ocimum tenuiflorum.
Ocimum basilicum having strong antibacterial and antioxidant properties are widely used for medicinal purposes. Leaves and flowering parts of ocimum basilicum are believed to be rich of different phytochemicals and are traditionally used as. Antibacterial activity of ocimum basilicum rehan leaf. Phytochemical screening and antibacterial activities of. Ocimum basilicum essential oil associated with existing standard antibiotics may increase their antibacterial activity, resulting in a synergistic activity. Secondary metabolites from ocimum species possess exceptional biological activity and have antioxidant 68 and antimicrobial 912, bactericidal 14, repellent 1516, anticonvulsant 17, chemopreventive and radioprotective effects 1819. The lamiaceae family is one of the most important families, numbering over 5,000 medicinal and aromatic plant species with extracted essential oils for multiple applications 1. Ocimum basilicum essential oil associated with existing standard antibiotics may increase their antibacterial activity, resulting in a synergistic activity against bacterial strains of clinical importance. It is medicinally used in case of diarrhea as well as hypoglycemic, antiinflammatory, antibacterial, antioxidant, immunestimulatory, and antiviral charvat et al. A total of 146 microbial organisms belonging to 55 bacteria, and four fungi, and a yeast species were studied using a diskdiffusion and minimal inhibition concentration mic method.
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