Paenibacillus alvei, paenibacillus gordonae, paenibacillus larvae, paenibacillus pulvifaciens, paenibacillus macerans, paenibacil ius azotojixans, paenibacillus pabuli, paenibacillus macquarien sis, paenibacillus amylolyticus, and paenibacillus validus. Paenibacillus is a genus of facultative anaerobic, endosporeforming bacteria, originally. Despite the difficulties in assigning to human paenibacillus isolates a role as. Six bacillus species including percentage of isolates in brackets bacillus licheniformis 67%, bacillus cereus 19%, bacillus thuringensis 4%, bacillus subtilis 4%, bacillus pumilus 4% and paenibacillus glucanolyticus 2% were identified based on blast databases in six different wpc80 batches.
Paenibacillus glucanolyticus, paenibacillus macquariensis, and. All the five paenibacillus species that showed negative results in pcr amplification belong to the same 16s rrnabased phylogenetic group xi. Pdf bacillus glucanolyticus, a new species that degrades a. Bacillus glucanolyticus, a new species that degrades a variety of b etaglucans. Hosted by the usgs core science analytics and synthesis. However, although generally considered nonpathogenic, most of the other species. The evaluation of toxinenzyme secretion, toxinencoding genes, motility, and biofilm formation revealed that b. Mar 14, 2019 paenibacillus m a taxonomic genus within the family paenibacillaceae certain facultative anaerobic, endospore forming bacteria. There is limited research on these lipopeptides, but they are diverse, numerous, and potent.
Origins of the invitro honeybee rearing methodology66. Originally derived from a bacillus, group 3 was proposed by ash et al. In this study, we evaluated the use of matrixassisted laser. Mar 31, 2016 in this study, we demonstrate that biofilm formation is a widespread virulence behavior of clinical bacillus and paenibacillus isolates. All lower taxonomy nodes 1 common name isynonym iother names i atcc 49278 bacillus glucanolyticus bacillus glucanolyticus alexander and priest 1989. Paenibacillus larvae causes american foulbrood in honey bees. Clos tridium durum has also been transferred to this genus 6.
The genus paenibacillus is characterized as rodshaped grampositive or gramvariable endospore forming aerobic or facultatively anaerobic bacteria. A welldesigned environmental monitoring programme should detect the presence of bacteria, filamentous fungi and yeasts before product contamination occurs. However, it is not a legal authority for statutory or regulatory purposes. An antimicrobial compound was extracted from paenibacillus osy. That such preparations may be contaminated with spores of this organism is not well known among pharmacists, physicians, and addicts. The soilrelated bacillus and paenibacillus species have increasingly been implicated in various human diseases. Pdf bacillus glucanolyticus, a new species that degrades. Complete genome sequence of paenibacillus strain y4. No product was detected with any nonpaenibacillus species and products of the appropriate size 240 bp were detected with 22 species of paenibacillus. Transfer of bacillus alginolyticus, bacillus chondroitinus, bacillus curdlanolyticus, bacillus glucanolyticus, bacillus kobensis, and bacillus thiaminolyticus to the genus paenibacillus and emended description of the genus paenibacillus. Paenibacillus curdlanolyticus strain b6 multienzyme.
Emended description of paenibacillus amylolyticus and. Paenibacillus glucanolyticus bacillus circulans, paenibacillus lautus, and bacillus megaterium were isolated from sub 75. This, combined with genomic cazyme analysis, suggests an intestinal, rather than environmental origin for y412mc10. Paenibacillus glucanolyticus ex alexander and priestet al. Moreover, most of the spores formed were found in the biofilms of paenibacillus glucanolyticus and paenibacillus polymyxa. Food and drug administration office of regulatory affairs. Ncbi taxonomy catalogue of life col species database global biodiversity information facility gbif biodiversity database global catalogue of microorganisms.
The sporeforming bacterium paenibacillus larvae is the agent behind american foulbrood afb, a key larval pathogen of the honey bee, apis mellifera. Phylum firmicutes, class bacilli, order bacillales, family paenibacillaceae, genus paenibacillus, paenibacillus massiliensis roux and raoult 2004. Bacillus glucanolyticus, a new species that degrades a. Comparison to other paenibacillus species shows the organism lacks nitrogen fixation, antibiotic production and social interaction genes reported in other paenibacilli. Young larvae from the first and second instars are highly susceptible to this disease, with the ingestion of as few as 10 infectious spores being sufficient to cause mortality. Isolated from a wide range of sources, the genus paenibacillus. The isolate was initially classified as a geobacillus sp. Paenibacillus glucanolyticus alexander and priest shida et. Current knowledge and perspectives of paenibacillus. Identification and pathogenic potential of clinical bacillus. The species paenibacillus glucanolyticus was originally described and validly published by shida et al. Y412mc10 based on its isolation conditions and similarity to other organisms isolated from hot springs at yellowstone national park. The bacterium paenibacillus glucanolyticus was isolated from black liquor p. Seventeen isolates were found to meet the phenotypic and genotypic criteria to be candidatus to 11 species of paenibacillus.
This culture exhibits a phenomenon known as motile microcolonies, which can. Itis taxonomy is based on the latest scientific consensus available, and is provided as a general reference source for interested parties. Bacillus glucanolyticus, a new species that degrades a variety of glucans. A new species, bacillus glucanolyticus, is proposed for a group of facultatively anaerobic endosporeforming bacteria isolated from soil that hydrolyze various. Although it is not a problem in its area of origin, the beetle causes serious damage in the usa. Paenibacillus glucanolyticus alexander and priest shida et al. Paenibacillus species have been isolated from a variety of sources including soil, fresh and. Nrrl nrs16 which have a common origin pci 221 and strain nrrl. Restriction patterns of pcramplified 16s rdna genes among paenibacillus, bacillus, brevibacillus, and virgibacillus species and origins of the strains used in. Paenibacillus urinalis 5402403 type strain dsm 22281. In addition, one isolate sent to weac for further bacterial. Y412mc10 was one of a number of organisms isolated from obsidian hot spring, yellowstone national park, montana, usa under permit from the national park service. Paenibacillus glucanolyticus alexander and priest 1989 shida et al. Food and drug administration office of regulatory affairs summary report for sample number.
Isolated from a wide range of sources, the genus paenibacillus comprises bacterial species relevant to humans, animals, plants, and the environment. Hgf5, an organism from the human microbiome project hmp reference genomes. Latin paene means almost, so paenibacilli are literally almost bacilli. Apr 12, 2018 paenibacillus glucanolyticus timelapse video showing growth over 80 hours at room temperature on standard methods agar. Dec 05, 2011 a new bacterial strain, designated osyse, which produces a unique and potent antimicrobial agent was isolated from soil. Grampositive, motile peritrichous flagella rods, 0. Gramnegative, sporeforming rods were isolated from a human urine sample strain 5402403t and a human cerebrospinal fluid sample strain 4401170t. Pronunciation of paenibacillus with 2 audio pronunciations, 1 translation and more for paenibacillus. Paenibacillus glucanolyticus alexander and priest shida.
Phylogenetic analysis based on 16s rrna gene sequence comparison showed that they clustered with paenibacillus massiliensis 2301065t. The y412mc10 genome shows a high level of synteny and homology to the draft sequence of paenibacillus sp. Strain v10537t was clearly distinguishable from other paenibacillus species and thus represents a novel species of the genus paenibacillus, for which the name paenibacillus taichungensis sp. Paenibacillus larvae bacteremia in injection drug users. Lastly, the spores derived from these biofilms were longer than those derived from planktonic cells, as observed by phase contrast microscopy. This result highlights the importance of catheter removal, commonly practiced in our hospital, in the case of deep bacillus or paenibacillus infections. Paenibacillus curdlanolyticus strain b6 multienzyme complex. The source of microbiological contamination of whey protein concentrate wpc, a quality problem for the dairy industry, has not been thoroughly investigated. Feb 29, 2012 identifying microbes in nonsterile facilities. While every effort has been made to provide the most reliable and uptodate information available, ultimate legal requirements with respect to species are contained in. Detection of eleven new species article pdf available may 2017 with 741 reads. Paenibacillus larvae is a species of bacterium, found worldwide, which causes american foulbrood, a fatal disease of the larvae of honeybees apis mellifera. Bacillus glucanolyticus, bacillus kobensis, and bacillus thiaminolyticus. Assessment of the diversity of paenibacillus species in.
Atcc 49278 bacillus glucanolyticus bacillus glucanolyticus alexander and priest 1989 ccug 28523 cip 106935 more dsm 5162 hscc 171 ifo 15330 lmg 12239 lmg. Paenibacillus glucanolyticus motile microcolonies youtube. Identification and pathogenic potential of clinical. Ncbi taxonomy catalogue of life col species database global biodiversity.
Bacillaceae natural habitats soil and feed, for cattle and other. Based on genotypic characteristics, these strains belonged to the genus paenibacillus and were closely related. We determined the taxonomic status of six bacillus species bacillus alginolyticus, bacillus chondroitinus, bacillus curdlanolyticus, bacillus glucanolyticus, bacillus kobensis, and bacillus thiaminolyticus by using the results of 16s rrna gene sequence and cellular fatty acid composition analyses. Paenibacillus produce a wide variety of lipopeptides that could be developed into antibacterial, antifungal, anticancer and antiviral drugs. Bacillus curdlanolyticus, bacillus glucanolyticus, bacillus kobensis, and. It is a grampositive, rodshaped bacterium, which forms spores which can remain viable for at least thirtyfive years. Characterisation and biofilm screening of the predominant. The objectives of this study were to identify the bacteria isolated from whey protein concentrate containing 80% protein wpc 80 and determine their ability to form biofilms as a potential source of contamination in a whey processing. Paenibacillus glucanolyticus timelapse video showing growth over 80 hours at room temperature on standard methods agar. Paenibacillus larvae is a sporeforming grampositive microorganism known for its ability to cause american foulbrood, a severe and noti.
A new bacterial strain, designated osyse, which produces a unique and potent antimicrobial agent was isolated from soil. Paenibacillus favisporus, paenibacillus glucanolyticus, paenibacillus glycanilyticus, paenibacillus gordonae, paenibacillus graminis, paenibacillus granivorans, paenibacillus hodogayensis. Bacteria belonging to this genus have been detected in a variety of environments, such as. Despite, the difficulties to assign to the human paenibacillus isolates a role as pathogen or contaminant, here 25% of the isolates were. Paenibacillus species are described as among a gold mine of antibiotic candidates. Shida o, takagi h, kadowaki k, nakamura lk, komagata k. Paenibacillus species paenibacillus glucanolyticus name synonyms bacillus glucanolyticus bacillus glucanolyticus alexander and priest 1989 paenibacillus glucanolyticus alexander and priest 1989 shida et al. Many paenibacillus species can promote crop growth directly via biological nitrogen fixation, phosphate solubilization, production of the phytohormone indole3acetic acid iaa, and release of siderophores that enable iron acquisition. Young larvae from the first and second instars are highly susceptible to this disease, with the ingestion of as. Paenibacillus is a genus of facultative anaerobic, endospore forming bacteria, originally included within the genus bacillus and then reclassified as a separate genus in 1993. Transfer of bacillus alginolyticus, bacillus chondroitinus. Despite the difficulties in assigning to human paenibacillus isolates a role as a. Paenibacillus polymyxa is an endosporeforming bacterium that is nonpathogenic and found in environments such as plant roots in soil and marine sediment 12. Growth on black liquor produced malonic, succinic, gallic, and.
Paenibacillus is a genus of facultative anaerobic, endosporeforming bacteria, originally included within the genus bacillus and then reclassified as a separate genus in 1993. Paenibacillus urinalis global catalogue of microorganisms. Phylogenetic analysis clustered these species closely with the paenibacillus species. A novel system for biomass utilization by khanok ratanakhanokchai, rattiya waeonukul, patthra pason, chakrit tachaapaikoon, khin lay kyu, kazuo sakka, akihiko kosugi and yutaka mori. Page designed through the cooperative efforts of interagency itis teams.
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